Publications

2017

Warda AS*, Kretschmer J*, Hackert P, Lenz C, Urlaub H, Höbartner C, Sloan KE, Bohnsack MT (2017) Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep 18, 2004-2014.

Bohnsack MT#, Sloan KE# (2017) The mitochondrial epitranscriptome: the roles of RNA modifications in mitochondrial translation and human disease. Cell Mol Life Sci, in press.

Memet I, Doebele C, Sloan KE#, Bohnsack MT# (2017) The G-patch protein NF-κB-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis. Nucleic Acids Res 45, 5359-5374.

Sloan KE, Warda AS, Sharma S, Entian KD, Lafontaine DL, Bohnsack MT (2017) Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function. RNA Biol, in press.

Haag S, Sloan KE, Höbartner C#, Bohnsack MT# (2017) In vitro assays for RNA methyltransferase activity. Meth Mol Biol 1562, 259-268.

Haag S, Kretschmer J, Sloan KE, Bohnsack MT (2017) Crosslinking methods to identify RNA methyltransferase targets in vivo. Meth Mol Biol 1562, 269-281.

Sloan KE, Höbartner C, Bohnsack MT (2017) How RNA modification allows non-conventional decoding in mitochondria. Cell Cycle 16, 145-146.

Jungfleisch J, Nedialkova D, Dotu I, Sloan KE, Martinez-Bosch N, Brüning L, Raineri E, Navarro P, Bohnsack MT, Leidel SA, Diez J (2017) A novel translational control mechanism involving RNA structures within coding sequences. Genome Res 27, 95-106. (Recommended as Research Highlight by Nature Reviews Molecular Cell Biology, doi:10.1038/nrm.2016.178)

 

2016

Warda AS, Freytag B, Haag S, Sloan KE, Görlich D, Bohnsack MT (2017) Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment. Hum Mol Genet 25, 5353-5364. (Selected for the cover of Human Molecular Genetics)

Haag S*, Sloan KE*, Ranjan N*, Warda AS*, Kretschmer J, Blessing C, Hübner B, Seikowski J, Dennerlein S, Rehling P, Rodnina MV, Höbartner C#, Bohnsack MT# (2016) NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation. EMBO J 35, 2104-2119. (Recommended by News & Views in EMBO Journal, DOI: 10.15252/embj.201695385; invited Feature in Cell Cycle, DOI: 10.1080/15384101.2016.1235860)

Heininger AU, Hackert P, Andreou AZ, Boon KL, Memet I, Prior M, Clancy A, Schmidt B, Urlaub H, Schleiff E, Sloan KE, Deckers M, Lührmann R, Enderlein J, Klostermeier D, Rehling P, Bohnsack MT (2016) Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol 13, 320-330.

Sloan KE, Gleizes PE, Bohnsack MT (2016) Nucleocytoplasmic transport of RNAs and RNA-protein complexes. J Mol Biol 428, 2040-2059.

 

2015

Haag S, Warda A, Kretschmer J, Günnigmann MA, Höbartner C, Bohnsack MT (2015) NSUN6 is a human RNA methyltransferase that catalyses formation of m5C72 in specific tRNAs. RNA 21, 1532-1543.

Sloan KE*, Leisegang MS*, Döbele C*, Ramirez AS, Simm S, Safferthal C, Schorge T, Markoutsa S, Kretschmer J, Haag S, Karas M, Ebersberger I, Schleiff E, Watkins NJ, Bohnsack MT (2015) The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21. Nucleic Acids Res 43, 553-564.

Weis BL, Palm D, Missbach S, Bohnsack MT, Schleiff E (2015) atBRX1-1 and atBRX1-2 are involved in an alternative rRNA processing pathway in Arabidopsis thaliana. RNA 21, 415-425.

Haag S, Kretschmer J, Bohnsack MT (2015) WBSCR22/Merm1 is required for late nuclear pre-ribosomal RNA processing and mediates N7-methylation of G1639 in human 18S rRNA. RNA 21, 180-187.

 

2014

Weis BL, Missbach S, Bohnsack MT, Schleiff E (2014) The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana. Plant J 80, 1043-1056.

Martin R, Hackert P, Ruprecht M, Simm S, Brüning L, Mirus O, Sloan KE, Kudla G, Schleiff E, Bohnsack MT (2014) A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase. RNA 20, 1173-1182.

Sloan KE, Bohnsack MT, Schneider C, Watkins NJ (2014) The roles of SSU Processome Components and Surveillance Factors in the Initial Processing of Human Ribosomal RNA. RNA 20, 540-550. 

Ebersberger I, Simm S, Leisegang MS, Mirus O, von Haeseler A, Bohnsack MT, Schleiff E (2014) The evolutionary history of the ribosome biogenesis pathway from a yeast perspective. Nucleic Acids Res 42, 1509-1523.

 

2013

Martin R*, Straub A*, Döbele C*, Bohnsack MT (2013) DExD/H-box RNA Helicases in Ribosome Biogenesis. RNA Biol 10, 4-18.

Sloan KE, Bohnsack MT, Watkins NJ (2013) The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress. Cell Rep 5, 237-247.

Missbach S, Weis BL, Martin R, Bohnsack MT, Schleiff E (2013) 40S ribosome biogenesis cofactors are essential for gametophyte and embryo development. PLoS One 8, e54084.

 

2012

Bohnsack MT, Tollervey D, Granneman S (2012) Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC). Methods in Enzymology 511, 275-288.

Veith T*, Martin R*, Wurm JP*, Weis BL, Duchardt-Ferner E, Safferthal C, Hennig R, Mirus O, Bohnsack MT, Wöhnert J, Schleiff E (2012) Structural and Functional Analysis of the Archaeal Endonuclease Nob1. Nucleic Acids Res 40, 3259-3274.

Watkins NJ, Bohnsack MT (2012) The box C/D and H/ACA snoRNPs: key players in the modification, processing and the dynamic folding of ribosomal RNA. Wiley Interdiscip Rev RNA 3, 397-414.

Leisegang MS*, Martin R*, Ramirez AS*, Bohnsack MT (2012) Exportin T and Exportin 5: tRNA and miRNA biogenesis - and beyond. Biol Chem 393, 599-604.

Veith T, Wurm JP, Duchardt-Ferner E, Weis B, Martin R, Safferthal C, Bohnsack MT, Schleiff E, Wöhnert J (2012) Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis. Biomol NMR Assign 6, 47-50.

 

2011

Meyer B, Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD (2011) The protein mutated in Bowen-Conradi Syndrome, Nep1 (Emg1), is required for a unique modification in 18S rRNA. Nucleic Acids Res 39, 1526-1537.

Lehmann P, Bohnsack MT, Schleiff E (2011) The functional domains of the unusual chloroplast positioning protein 1. Plant Science 180, 650-654.

Lehmann P, Nöthen J, Schmidt von Braun S, Bohnsack MT, Mirus O, Schleiff E (2011) Transitions of gene expression induced by short-term blue light. Plant Biol 13, 349-361.

 

Publications until 2010

Bohnsack MT, Schleiff E (2010) The evolution of protein targeting and translocation systems. Biochim Biophys Acta 1803, 1115-1130.

Bohnsack MT#, Martin R, Granneman S, Ruprecht M, Schleiff E, Tollervey D# (2009) Prp43 bound at different sites on the Pre-rRNA performs distinct functions in ribosome synthesis. Mol Cell 36, 583-592.   # Corresponding author.  (Recommended by Preview in Molecular Cell, DOI 10.1016/j.molcel.2009.11.004)

Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE (2009) Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects. Hum Mol Genet 18, 3615-3625.

Bohnsack MT, Kos M, Tollervey D (2008) Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase. EMBO Rep 9, 1230-1236.

Ciais D, Bohnsack MT, Tollervey D (2008) The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3’ processing pathway. Nucleic Acids Res 36, 3075-3084.

Leulliot N*, Bohnsack MT*, Graille M, Tollervey D, Van Tilbeurgh H (2008) The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Nucleic Acids Res 36, 629-639.   * Equal contribution.

Dumont J, Petri S, Pellegrin F, Terret ME, Bohnsack MT, Rassinier P, Georget V, Kalab P, Gruss OJ, Verlhac MH (2007) A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes. J Cell Biol 176, 295-305.

Bredemeier R, Schlegel T, Ertel F, Vojta A, Borissenko L, Bohnsack MT, Groll M, von Haeseler A, Schleiff E (2007) Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. J Biol Chem 282, 1882-1890.

Ribbeck K, Groen AC*, Santarella R*, Bohnsack MT*, Raemaekers T*, Köcher T, Gentzel M, Görlich D, Wilm M, Carmeliet G, Mitchison TJ, Ellenberg J, Hoenger A, Mattaj IW (2006) NuSAP, a mitotic RanGTP target that stabilizes and Cross-links microtubules. Mol Biol Cell 17, 2646-2660.

Bohnsack MT, Stüven T, Cordes V, Görlich D (2006) A selective block of nuclear actin export stabilises the giant nuclei of Xenopus oocytes. Nat Cell Biol 8, 257-263. (Recommended on F1000prime, DOI: 10.3410/f.1030122.368882)

Mingot JM, Bohnsack MT, Jäkle U, Görlich D (2004) Exportin 7 defines a novel general nuclear export pathway. EMBO J 23, 3227-3236.

Bohnsack MT*, Czaplinski K*, Görlich D (2004) Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA 10, 185-191.

Bohnsack MT, Regener K, Schwappach B, Saffrich R, Paraskeva E, Hartmann E, Görlich D (2002) Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. EMBO J 21, 6205-6215. (Recommended by 4 Members of F1000prime, see e.g. DOI: 10.3410/f.1010373.153907)