Prof. Dr. Peter Rehling

Universitätsmedizin Göttingen
Institut für Zellbiochemie
Humboldtallee 23
D-37073 Göttingen

Secretary: Eva Ausmeier

Phone: +49 (0)551 39-65947
Fax: +49 (0)551 39-5979
E-mail: eva.ausmeier(at)med.uni-goettingen.de

1991 – 1993 Diploma work in the lab of Prof. Dr. E.W. Weiler, Ruhr-Universität-Bochum.

1993 – 1996 PhD thesis in the lab of Prof. Dr. Dr. W.-H. Kunau at the Institut für Physiologische Chemie, Ruhr University Bochum.

1996 – 1998 Postdoctoral position in the lab of Prof. Dr. Dr. W.-H. Kunau.

1998 – 2000 Postdoctoral position in the lab of Prof. Dr. S.D. Emr, Howard Hughes Medical Institute and Division of Cellular & Molecular Medicine, University of California San Diego, USA.

2000 – 2004 Assistant professor (Habilitation) at the Institute for Biochemistry and Molecular Biology, University of Freiburg.

2001 – 2006 Group leader in the SFB388 Zelluläre Funktionen dynamischer Proteinwechselwirkungen.

2003 Habilitation for Biochemistry and Molecular Biology at the Medical Faculty, University of Freiburg.

since 2007 Head of the Department of Cellular Biochemistry (formerly Biochemistry II) of the University of Göttingen

2010 – Max Planck Institute for Biophysical Chemistry Research Fellow

2015 – Program Director International Max Planck Research School “Molecular Biology”

2016 – Coordinator Collaborative Research Center on “Compartmental Gates and Contact Sites in cells” (SFB1190)

2004 Research Award of the University of Freiburg

2005 Young Investigator Award of the GBM and Schering Stiftung

2013 ERC Advanced Grant „Mitochondrial translational regulation coupled to respiratory chain assembly and protein import“ – MITRAC”

2016 Copernicus Award of the DFG and FNP together with Prof. Dr. A. Chacinska

Mitochondria are the powerhouse of eukaryotic cells and produce the bulk of cellular ATP through oxidative phophorylation. Moreover, mitochondria fulfil additional important cellular tasks such as the generation of FeS-clusters, they are involved in amino acid and lipid metabolism, and the regulation of programmed cell death.

Most of the approximately 1000 mitochondrial proteins are imported from the cytosol into mitochondria post-translationally. However, a small number of hydrophobic proteins are synthesized within mitochondria.

We are interested in understanding the molecular mechanisms by which proteins are transported across the mitochondrial membranes and to find out how multi-protein complexes in the inner membrane (TIM complexes; translocation machineries of the inner membrane) mediate this task. In another aspect of our work we addresses the question as to how newly imported proteins assemble into multi-protein complexes in the inner membrane. In case of the respiratory chain complexes the assembly process is especially demanding since central subunits of the complexes are made within mitochondria. Dedicated chaperone-like factors are required to assist and regulate the assembly process. The analysis of the principles of the biogenesis process and the activities of the assembly factors is of central importance for our understanding of the molecular basis of human mitochondrial disorders. In our work we combine biochemical and genetic techniques on the model organism Saccharomyces cerevisae with experiments in human cell lines. Research topics that we currently address are:

Transport and membrane insertion of mitochondrial proteins.

Assembly of inner mitochondrial membrane complexes and how this process is affected in mitochondrial disorders.

Biogenesis of mitochondrially-encoded proteins.

Mitochondrial translational regulation coupled to respiratory chain assembly and protein import

Mitochondrial respiratory chain complexes assemble from nuclear- and mitochondria-encoded subunits in the inner membrane. To protect cells from accumulating unassembled subunits in the membrane, ROS generation, or damage of mitochondrial integrity, regulatory mechanisms to balance and control the fidelity of the assembly process have evolved, encorporating a retention system enabling the preservation of a select few founding complexes for on-demand assembly. In yeast mitochondria, a feedback mechanism has been identified for the cytochrome oxidase in which assembly-intermediates specifically inactivate translation of the core subunit Cox1. It is controversially debated if similar translation regulating mechanisms exist in human mitochondria. However, our recent findings show that in human mitochondria assembly intermediates of respiratory chain complexes affect translation, nevertheless the underlying sensing and signalling mechanisms as well as the protein components appear to be more complex than in yeast. We aim to understand how this regulatory cycle is established; how does a distinct assembly intermediate of cytochrome oxidase signal the translation system, and how the influx of imported subunits contributes to this process. These goals are conceptually deeply rooted in a comprehensive understanding of the membrane protein complex assembly process and the factors that promote its progression. These objectives are of key importance for understanding the molecular pathology of mitochondrial encephalomyopathies that are frequently due to respiratory chain assembly and quality control malfunction. The aim of our analyses is to provide insight as to how translation can be coupled to the assembly of a membrane protein complex comprised of subunits of dual genetic origin and to decipher mitochondrial translational regulation coupling to the influx of imported nuclear encoded subunits.

  • Dennerlein, S., Oeljeklaus, S., Jans, D., Hellwig, C., Bareth, B., Jakobs, S., Deckers, M., Warscheid, B., Rehling, P. (2015), MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly. Cell Rep. [Epub ahead of print]
  • Pacheu-Grau, P., Bareth, B., Dudek, J., Juris, L., Vögtle, F.-N., Wissel, M., Leary, S., Dennerlein, S., Rehling, P., Deckers, M. (2015), Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies. Cell Metabolism 21, 823-833
  • Dennerlein, S., Rehling, P. (2015), Human mitochondrial COX1 assembly into cytochrome c oxidase at a glance. J. Cell Sci. 128, 833-837

The lab is always interested in applications of highly motivated PhD students and Postdoctoral fellows who are interested in exciting molecular aspects of mitochondrial cell biology.

Applicants should have a strong background in biochemistry, chemistry, or biology. We expect that applicants have previously worked with human cell culture and are familiar with the related techniques. Since the project will aim for generating and analyzing mouse models, experience in working with mice would be instrumental to the project. We are looking for a ambitious coworkers to work as part of our international team in Göttingen.

Please send application with a cover letter (describing your interests and motivation), a full CV, copies of exams, and two letters of reference as well as a list of publications (in case of Postdoctoral fellows).

1. Protein dynamics of protein translocases during precursor transport

2. Mechanisms of translational regulation in mitochondria – a molecular basis for human disorders

Recent publications of our work are listed on the Webpage

For further questions, please do not hesitate to contact Peter Rehling

2020

Linden A., Deckers M., Parfentev I., Pflanz R., Homberg B., Neumann P., Ficner R., Rehling P., Urlaub H. (2020) A Cross-Linking Mass Spectrometry Approach Defines Protein Interactions in Yeast Mitochondria. Mol Cell Proteomics 2020 Apr 24;mcp.RA120.002028. doi: 10.1074/mcp.RA120.002028. Online ahead of print.

Stephan T., Brüser C., Deckers M., Steyer AM., Balzarotti F., Barbot M., Behr TS., Heim G., Hübner W., Ilgen P., Lange F., Pacheu-Grau D., Pape JK., Stoldt S., Huser T., Hell SW., Möbius W., Rehling P., Riedel D., Jakobs S. (2020) MICOS Assembly Controls Mitochondrial Inner Membrane Remodeling and Crista Junction Redistribution to Mediate Cristae Formation. EMBO J. 2020 Jun 22;e104105. doi: 10.15252/embj.2019104105. Online ahead of print.

Gomkale R., Cruz-Zaragoza LD., Suppanz I., Guiard B., Montoya J., Callegari S., Pacheu-Grau D., Warscheid B., Rehling P. (2020) Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos. Curr Biol. 2020 Jan 23. pii: S0960-9822(20)30024-5. doi: 10.1016/j.cub.2020.01.024. [Epub ahead of print]

Callegari S., Cruz-Zaragoza LD., Rehling P. (2020) From TOM to the TIM23 complex – handing over of a precursor. Biol Chem. 2020 Feb 1. pii: /j/bchm.just-accepted/hsz-2020-0101/hsz-2020-0101.xml. doi: 10.1515/hsz-2020-0101. [Epub ahead of print]

Pacheu-Grau D., Wasilewski M., Oeljeklaus S., Gibhardt CS., Aich A., Chudenkova M., Dennerlein S., Deckers M., Bogeski I., Warscheid B., Chacinska A., Rehling P. (2020) COA6 facilitates cytochrome c oxidase biogenesis as thiol-reductase for copper metallochaperones in mitochondria. J Mol Biol. 2020 Feb 13. pii: S0022-2836(20)30102-9. doi: 10.1016/j.jmb.2020.01.036

Wang C., Richter-Dennerlein R., Pacheu-Grau D., Liu F., Zhu Y., Dennerlein S., Rehling P. (2020) MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex. EMBO Rep. 2020 Jan 7;21(1):e48833. doi: 10.15252/embr.201948833. Epub 2019 Nov 13.

2019

Zhang X., Gibhardt CS., Will T., Stanisz H., Körbel C., Mitkovski M., Stejerean I., Cappello S., Pacheu-Grau D., Dudek J., Tahbaz N., Mina L., Simmen T., Laschke MW., Menger MD., Schön MP., Helms V., Niemeyer BA., Rehling P., Vultur A, Bogeski I. (2019) Redox signals at the ER-mitochondria interface control melanoma progression. EMBO J. 38:e100871

Callegari S., Rehling P. (2019) Guarding the gate for mitochondrial entry. Nature 569, 635-637

Can K., Menzfeld C., Rinne L., Rehling P., Kügler S., Golubiani G., Dudek J., Müller M. (2019) Neuronal Redox-Imbalance in Rett Syndrome Affects Mitochondria as Well as Cytosol, and Is

Accompanied by Intensified Mitochondrial O2 Consumption and ROS Release. Front Physiol 10:479

Callegari S., Müller T., Schulz C., Lenz C., Jans DC., Wissel M., Opazo F., Rizzoli SO., Jakobs S., Urlaub H., Rehling P., Deckers M. (2019) A MICOS-TIM22 Association Promotes Carrier Import into Human Mitochondria. J Mol Biol. 431, 2835-2851

Mohanraj K., Wasilewski M., Benincá C., Cysewski D., Poznanski J., Sakowska P., Bugajska Z., Deckers M., Dennerlein S., Fernandez-Vizarra E., Rehling P., Dadlez M., Zeviani M., Chacinska, A. (2019) Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO Mol. Med. 11(5). pii: e9561

Richter F., Dennerlein S., Nikolov M., Jans DC., Naumenko N., Aich A., MacVicar T., Linden A., Jakobs S., Urlaub H., Langer T., Rehling P. ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. (2019) J Cell Biol. 218, 598-614

Antunes D., Chowdhury A., Aich A., Saladi S., Harpaz N., Stahl M., Schuldiner M., Herrmann JM., Rehling P., Rapaport D. (2019) Overexpression of branched-chain amino acid aminotransferases rescues the growth defects of cells lacking the Barth syndrome-related gene TAZ1. J Mol Med 97, 269-279

Nanadikar MS., Vergel Leon AM., Borowik S., Hillemann A., Zieseniss A., Belousov VV., Bogeski I., Rehling P., Dudek J., Katschinski DM. (2019) O2 affects mitochondrial functionality ex vivo. Redox Biol. 22, 101152

Dudek J., Hartmann M., Rehling P. (2019) The role of mitochondrial cardiolipin in heart function and its implication in cardiac disease. Biochim Biophys Acta Mol Basis Dis. 1865, 810-821

2018

Saal KA., Richter F., Rehling P., Rizzoli SO. (2018) Combined Use of Unnatural Amino Acids Enables Dual Color Super-Resolution Imaging of Proteins via Click Chemistry. ACS Nano ACS Nano 12, 12247–12254

Pacheu-Grau D., Callegari S., Emperador S., Thompson K., Aich A., Topol SE., Spencer E.G., McFarland R., Ruiz-Pesini E., Torkamani A., Taylor R.W., Montoya J., Rehling P. (2018) Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy. Hum Mol Genet. 27 (23), 4135-4144

Mandad S., Rahman R.U., Centeno T.P., Vidal R.O., Wildhagen H., Rammner B., Keihani S., Opazo F., Urban I., Ischebeck T., Kirli K., Benito E., Fischer A., Yousefi R.Y., Dennerlein S., Rehling P., Feussner I., Urlaub H., Bonn S., Rizzoli S.O., Fornasiero E.F. (2018) The codon sequences predict protein lifetimes and other parameters of the protein life cycle in the mouse brain. Sci Rep. 8 (1),16913

Fornasiero E.F., Mandad S., Wildhagen H., Alevra M., Rammner B., Keihani S., Opazo F., Urban I., Ischebeck T., Sakib M.S., Fard M.K., Kirli K., Centeno T.P., Vidal R.O., Rahman R.U., Benito E., Fischer A., Dennerlein S., Rehling P., Feussner I., Bonn S., Simons M., Urlaub H., Rizzoli S.O. (2018) Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. Nat Commun. 9, 4230

Chowdhury A., Aich A., Jain G., Wozny K., Lüchtenborg C., Hartmann M., Bernhard O., Balleiniger M., Alfar E.A., Zieseniss A., Toischer K., Guan K., Rizzoli S.O., Brügger B., Fischer A., Katschinski D.M., Rehling P., Dudek J. (2018) Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1a Expression in Hypoxia. Cell Reports 25, 561–570

Schendzielorz, A.B., Bragoszewski, P., Naumenko, N., Gomkale, R., Schulz, C., Guiard, B., Chacinska, A., Rehling, P. (2018) Motor recruitment to the TIM23 channel’s lateral gate restricts polypeptide release into the inner membrane. Nat Commun. 9, 4028

Aich, A., Wang, C., Chowdhury, A., Ronsör, C., Pacheu-Grau, D., Richter-Dennerlein, R., Dennerlein, S., Rehling P. (2018) COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis. Elife. 7. pii: e32572. doi: 10.7554/eLife.32572. [Epub ahead of print]

Lorenzi, I., Oeljeklaus, S., Aich, A., Ronsör, C., Callegari, S., Dudek, J., Warscheid, B., Dennerlein, S., Rehling, P. (2018) The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis. Biochim Biophys Acta. 1865, 323-333

Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D’Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, Richter F, Coy-Vergara, J., Coceano, G., Boyden, ES., Duncan, RR., Hell, SW., Lauterbach, MA., Lehnart, SE., Moser, T., Outeiro, TF., Rehling, P., Schwappach, B., Testa, I., Zapiec, B., Rizzoli, SO. (2018) Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy. EMBO J. 37, 139-159

2017

Naumenko, N., Morgenstern, M., Rucktäschel, R., Warscheid, B., Rehling, P. (2017) INA complex liaises the F1Fo-ATP synthase membrane motor modules. Nat Commun. 8, 1237

Callegari, S., Oeljeklaus, S., Warscheid, B., Dennerlein S., Thumm, M., Rehling, P.*, and Dudek, J*. (2017) Phospho-ubiquitin-Parkin complex as a marker for mitophagy signaling. Autophagy 13: 201-211.

Guentsch, A., Beneke, A., Swain, L., Farhat, K., Nagarajan, S., Wielockx, B., Dudek, J., Rehling, P., Zieseniss, A., Jatho, A., Chong, M., Santos, C., Shah, A.M., and Katschinski, D.M. (2017) PHD2 is a regulator for glycolytic reprogramming in macrophages. Mol. Cell. Biol. 37, 1-17

Schendzielorz, A.B., Schulz, C., Lytovchenko, O., Clancy, A., Guiard, B., Ieva, R., van der Laan, M., and Rehling, P. (2017) Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation. J. Cell Biol. 216:83-92

Denkert, N., Schendzielorz, A.B., Barbot, M., Versemann, L., Richter, F., Rehling, P.*, and Meinecke, M.* (2017) Cation selectivity of the presequence translocase channel Tim23 is crucial for efficient protein import. eLife Aug 31;6. pii: e28324. doi: 10.7554/eLife.28324.

Dennerlein, S., Wang, C., and Rehling, P. (2017) Plasticity of mitochondrial translation. Trends Cell. Biol. 27: 712–721

2016

Richter-Dennerlein, R., Oeljeklaus, S., Lorenzi, I., Ronsör, C., Bareth, B., Schendzielorz, AB., Wang, C., Warscheid, B., Rehling, P., Dennerlein, S. (2016) Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein. Cell 167, 471-483

Lorenzi, I., Oeljeklaus, S., Ronsör, C., Bareth, B., Warscheid, B., Rehling, P., Dennerlein, S. (2016) The ribosome-associated Mba1 escorts Cox2 from insertion machinery to maturing assembly intermediates. Mol Cell Biol. 36, 2782–2793

Bareth, B., Nikolov, M., Lorenzi, I., Hildenbeutel, M., Mick, D.U., Helbig, C., Urlaub, H., Ott, M., Rehling, P., Dennerlein, S. (2016) Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Mol Biol Cell 27, 1570-80

Levchenko, M., Römpler, K., Wuttke, J.M., Schmidt, B., Neifer, K., Juris, L., Wissel, M. Rehling, P., Deckers, M. (2016) Cox26 is a novel stoichiometric subunit of the yeast cytochrome c oxidase. BBA Mol. Cell Res. 1863, 1624-32

Heininger, A. U., Hackert, P., Andreou, A. Z., Boon, K. L., Memet, I., Prior, M., Clancy, A., Schmidt, B., Urlaub, H., Schleiff, E., Sloan, K. E., Deckers, M., Lührmann, R., Enderlein, J., Klostermeier, D., Rehling, P., Bohnsack, M. T. (2016) Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol, 13, 320-30.

Dudek, J., Cheng, I.F., Chowdhury, A., Wozny, K., Balleininger, M., Reinhold, R., Grunau, S., Callegari, S., Toischer, K., Wanders, R.J., Hasenfuß, G., Brügger, B., Guan, K., Rehling, P. (2015) Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome. EMBO Mol Med. 8, 139-54

Callegari, S., Richter, F., Chojnacka, K., Jans, DC., Lorenzi, I., Pacheu-Grau, D., Jakobs, S., Lenz, C., Urlaub, H., Dudek, J., Chacinska, A., Rehling, P. (2016) TIM29 is a subunit of the human carrier translocase required for protein transport. FEBS Lett. 590, 4147-4158

Haag, S., Sloan, KE., Ranjan, N., Warda, AS., Kretschmer, J., Blessing, C., Hübner, B., Seikowski, J., Dennerlein, S., Rehling, P., Rodnina, MV., Höbartner, C., Bohnsack, MT. (2016) NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation. EMBO J. 35, 2104-2119.

2015

Richter-Dennerlein, R., Dennerlein, S., Rehling, P. (2015) Integrating mitochondrial translation into the cellular context. Nat. Rev. Mol. Cell Biol. 16, 586-92

Dennerlein, S., Oeljeklaus, S., Jans, D., Hellwig, C., Bareth, B., Jakobs, S., Deckers, M., Warscheid, B., Rehling, P. (2015), MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly. Cell Rep. 12, 1644-1655

Pacheu-Grau, P., Bareth, B., Dudek, J., Juris, L., Vögtle, F.-N., Wissel, M., Leary, S., Dennerlein, S., Rehling, P., Deckers, M. (2015), Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies. Cell Metabolism 21, 823-833

Melin, J., Kilisch, M., Neumann, P., Lytovchenko, O., Gomkale, R., Schendzielorz, A., Schmidt, B., Liepold, T., Ficner, R., Jahn, O., Rehling, P., Schulz, C. (2015), A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochim. Biophys. Acta 1850, 1850-9

Schulz, C., Schendzielorz, A., Rehling, P. (2015), Unlocking the presequence import pathway. Trends Cell. Biol. 25, 265-275

Dennerlein, S., Rehling, P. (2015), Human mitochondrial COX1 assembly into cytochrome c oxidase at a glance. J. Cell Sci. 128, 833-837

2014

Schulz, C., Rehling, P. (2014), Cell Biology. Powering the cell cycle. Science 346, 1059-1060

Gornicka, A., Bragoszewski, P., Chroscicki, P., Wenz, L.S., Schulz, C., Rehling, P., Chacinska, A. (2014), A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria. Mol. Biol. Cell 25, 3999-4009

Lytovchenko, O., Naumenko, N., Oeljeklaus, S., Schmidt, B., von der Malsburg, K., Deckers, M., Warscheid, B., van der Laan, M., Rehling, P. (2014), The INA complex facilitates assembly of the peripheral stalk of the mitochondrial F1Fo-ATP synthase. EMBO J. 33, 1624-38

Pfanner, N., van der Laan, M., Amati, P., Capaldi, R. A., Caudy, A. A., Chacinska, A., Darshi, M., Deckers, M., Hoppins, S., Icho, T., Jakobs, S., Ji, J., Kozjak-Pavlovic, V., Meisinger, C., Odgren, P. R., Park, S. K., Rehling, P., Reichert, A. S., Sheikh, M. S., Taylor, S. S., Tsuchida, N., van der Bliek, A. M., van der Klei, I. J., Weissman, J. S., Westermann, B., Zha, J., Neupert, W., Nunnari, J. (2014), Uniform nomenclature for the mitochondrial contact site and cristae organizing system. J. Cell. Biol. 204, 1083-1086

Melin, J., Schulz, C., Wrobel, L., Bernhard, O., Chacinska, A., Jahn, O., Schmidt, B., Rehling, P. (2014), Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix. Mol. Cell Biol. 34, 3473-85

Schulz, C., Rehling, P. (2014), Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation.. Nat. Commun. 5, 4349

2013

Dudek, J., Rehling, P., van der Laan, M. (2013), Mitochondrial protein import: Common principles and physiological networks. Biochim. Biophys. Acta 1833, (2), 274-285

Bareth, B., Dennerlein, S., Mick, D.U., Nikolov, M., Urlaub, H., Rehling, P. (2013), The heme a synthase Cox15 associates with cytochrome c oxidase assembly intermediates during Cox1 maturation. Mol. Cell. Biol. 33, 4128-4137

Welter, E., Montino, M., Reinhold, R., Schlotterhose, P., Krick, R., Dudek, J., Rehling, P., Thumm ,M. (2013), Uth1 is a mitochondrial inner membrane protein dispensable for post-log phase and rapamycin-induced mitophagy. FEBS J. 280, 4970-4982

Dudek, J., Cheng, I.F., Balleininger, M., Vaz, F.M., Streckfuss-Bömeke, K., Hübscher, D., Vukotic, M., Wanders, R.J., Rehling, P., Guan, K. (2013), Cardiolipin deficiency affects respiratory chain function and organization in an induced pluripotent stem cell model of Barth syndrome. Stem Cell Res. 11, 806-819

Jans, D.C., Wurm, C.A., Riedel, D., Wenzel, D., Stagge, F., Deckers, M., Rehling, P., Jakobs, S. (2013), STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria. Proc Natl Acad Sci U S A. 110, 8936-8941

Lytovchenko, O., Melin, J., Schulz, C., Kilisch, M., Hutu, D.P., Rehling, P. (2013), Signal recognition initiates reorganization of the presequence translocase during protein import. EMBO J. 32, 886-898

2012

Vukotic, M., Oeljeklaus, S., Wiese, S., Vögtle, F.N., Meisinger, C., Meyer, H.E., Zieseniss, A., Katschinski, D.M., Jans, D.C., Jakobs, S., Warscheid, B., Rehling, P., Deckers, M. (2012), Rcf1 Mediates Cytochrome Oxidase Assembly and Respirasome Formation, Revealing Heterogeneity of the Enzyme Complex. Cell Metab. 15, (3), 336-347

Keil, M., Bareth, B., Woellhaf, M.W., Peleh, V., Prestele, M., Rehling, P., Herrmann, J.M. (2012), Oxa1-ribosome complexes coordinate the assembly of cytochrome c oxidase in mitochondria. J. Biol. Chem. 287, (41), 34484-34493

Reinhold, R., Krüger, V., Meinecke, M., Schulz, C., Schmidt, B., Grunau, S.D., Guiard, B., Wiedemann, N., van der Laan, M., Wagner, R., Rehling, P., Dudek, J. (2012), The channel-forming Sym1 protein is transported by the TIM23 complex in a presequence-independent manner. Mol. Cell. Biol. 32, (24), 5009-5021

Krüger, V., Deckers, M., Hildenbeutel, M., van der Laan, M., Hellmers, M., Dreker, C., Preuss, M., Herrmann, J.M., Rehling, P., Wagner, R., Meinecke, M. (2012), The mitochondrial oxidase assembly protein1 (Oxa1) insertase forms a membrane pore in lipid bilayers. J. Biol. Chem. 287, 33314-33326

Mick, D. U., Dennerlein, S., Wiese, H, Reinhold, R., Pacheu-Grau, D., Lorenzi, I., Sasarman, F., Weraarpachai, W., Shoubridge, E. A., Warscheid, B. and Rehling, P. (2012), MITRAC Links Mitochondrial Protein Translocation to Respiratory-Chain Assembly and Translational Regulation. Cell 151, 1528–1541

2011

Reinhold, R., Bareth, B., Balleininger, M., Wissel, M., Rehling, P., Mick, D.U. (2011), Mimicking a SURF1 allele reveals uncoupling of cytochrome c oxidase assembly from translational regulation in yeast. Hum. Mol. Genet. 20, (12), 2379-2393

Rehling, P. (2011), Tinkering with nature. Nat. Rev. Mol. Cell Biol. 12, (7), 401

Mick, D.U., Fox, T.D., Rehling, P. (2011), Inventory control: cytochrome c oxidase assembly regulates mitochondrial translation. Nat. Rev. Mol. Cell Biol. 12, 14-20

Lupo, D., Vollmer, C., Deckers, M., Mick, D.U., Tews, I., Sinning, I., Rehling, P. (2011), Mdm38 is a 14-3-3-like receptor and associates with the protein synthesis machinery at the inner mitochondrial membrane. Traffic 12, (10), 1457-1466

von der Malsburg, K., Müller, J.M., Bohnert, M., Oeljeklaus, S., Kwiatkowska, P., Becker, T., Loniewska-Lwowska, A., Wiese, S., Rao, S., Milenkovic, D., Hutu, D.P., Zerbes, R.M., Schulze-Specking, A., Meyer, H.E., Martinou, J.C., Rospert, S., Rehling, P., Meisinger, C., Veenhuis, M., Warscheid, B., van der Klei, I.J., Pfanner, N., Chacinska, A., van der Laan, M. (2011), Dual Role of Mitofilin in Mitochondrial Membrane Organization and Protein Biogenesis. Dev. Cell. 21, (4), 694-707

Schulz, C., Lytovchenko, O., Melin, J., Chacinska, A., Guiard, B., Neumann, P., Ficner, R., Jahn, O., Schmidt, B., Rehling, P. (2011), Tim50’s presequence receptor domain is essential for signal driven transport across the TIM23 complex. J. Cell. Biol. 195, 643-656

Gebert, N., Gebert, M., Oeljeklaus, S., von der Malsburg, K., Stroud, D.A., Kulawiak, B., Wirth, C., Zahedi, R.P., Dolezal, P., Wiese, S., Simon, O., Schulze-Specking, A., Truscott, K.N., Sickmann, A., Rehling, P., Guiard, B., Hunte, C., Warscheid, B., van der Laan, M., Pfanner, N., Wiedemann, N. (2011), Dual Function of Sdh3 in the Respiratory Chain and TIM22 Protein Translocase of the Mitochondrial Inner Membrane. Mol. Cell 44, (5), 811-818

2010

Van der Laan, M., Hutu, D.P., Rehling, P. (2010), On the mechanism of preprotein import by the mitochondrial presequence translocase. Biochim. Biophys. Acta 1803, (6), 732-739

Chacinska, A., van der Laan, M., Mehnert, C.S., Guiard, B., Mick, D.U., Hutu, D.P., Truscott, K.N., Wiedemann, N., Meisinger, C., Pfanner, N., and Rehling, P. (2010), Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol. Cell. Biol. 30, (1), 307-318

Bauerschmitt, H., Mick, D.U., Deckers, M., Vollmer, C., Funes, S., Kehrein, K., Ott, M., Rehling, P., and Herrmann, J.M. (2010), Ribosome Binding Proteins Mdm38 and Mba1 Display Overlapping Functions for Regulation of Mitochondrial Translation. Mol. Biol. Cell 21, (12), 1937-1944

Rehling, P., Rospert, S. (2010), Molecular chaperones and intracellular protein transport. Biochim. Biophys. Acta 1803, (6), 639-640

Bohnert, M., Rehling, P., Guiard, B., Herman, J.M., Pfanner, N., van der Laan, M. (2010), Cooperation of stop-transfer and conservative sorting mechanisms in mitochondrial protein transport. Curr. Biol. 20, 1227-1232

Mick, D.U., Vukotic, M., Piechura, H., Meyer, H.E., Warscheid, B., Deckers, M., Rehling, P. (2010), Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria. J. Cell Biol. 191, (1), 141-154

2009

Odorizzi, G. and Rehling, P. (2009), Membranes and organelles. Curr. Opin. Cell Biol. 21, 481-483

Wagner, K., Mick, D.U., and Rehling, P. (2009), Protein transport machineries for precursor translocation across the inner mitochondrial membrane. Biochim. Biophys.Acta. 1793, 52-59

Wagner, K., Rehling, P., Szklarz, L.K., Taylor, R.D., Pfanner, N., and van der Laan, M. (2009), Mitochondrial F1Fo-ATP synthase: the small subunits e and g associate with monomeric complexes to trigger dimerization. J. Mol. Biol. 392, (4), 855-861

Gebert, N., Joshi, A.S., Kutik, S., Becker, T., McKenzie, M., Guan, X.L., Mooga, V.P., Stroud, D.A., Kulkarni, G., Wenk, M.R., Rehling, P., Meisinger, C., Ryan, M.T., Wiedemann, N., Greenberg, M.L., Pfanner, N. (2009), Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome. Curr. Biol. 19, (24), 2133-2139

2008

Kutik, S., Rissler, M., Guan, X.L., Guiard, B., Shui, G., Gebert, N., Heacock, P.N., Rehling, P., Dowhan, W., Wenk, M.R., Pfanner, N. and Wiedemann, N. (2008), The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesis. J. Cell Biol. 183, 1213-1221

Gebert, N., Chacinska, A., Wagner, K., Guiard, B., Koehler, C.M., Rehling, P., Pfanner, N., and Wiedemann, N. (2008), Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep. 9, 548-554

Stuart, R. A. and Rehling, P. (2008), Mitochondrial biogenesis: is an old dog still teaching us new tricks?. EMBO Rep. 9, 33-38

Hutu, D. P., Guiard, B., Chacinska, A., Becker, D., Pfanner, N., Rehling, P., and Van der Laan, M. (2008), Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase. Mol. Biol. Cell 19, 2642-2649

Wagner, K., Gebert, N., Guiard, B., Brandner, K., Truscott, K.N., Wiedemann, N., Pfanner, N., and Rehling, P. (2008), The Assembly Pathway of the Mitochondrial Carrier Translocase Involves four Preprotein Translocases. Mol. Cell. Biol. 28, 4251-4260

2007

Reinders, J., Wagner, K., Zahedi, R.P., Stojanovski, D., Eyrich, B., Van der Laan, M., Rehling, P., Sickmann, A., Pfanner, N., and Meisinger, C. (2007), Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol. Cell. Proteomics 6, 1896-1906

Mick, D. U., Wagner, K., Van der Laan, M., Frazier, A. E., Perschil, I., Pawlas, M., Meyer, H.E., Warscheid, B., and Rehling, P. (2007), Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J. 26, 4347-4358

Van der Laan, M., Meinecke, M., Dudek, J., Hutu, D. P., Lind, M., Perschil, I., Guiard, B., Wagner, R., Pfanner, N., and Rehling, P. (2007), Motor-free mitochondrial presequence translocase drives membrane integration of preproteins. Nat. Cell. Biol. 9, 1152-1159

Wiedemann N., van der Laan M., Hutu D.P., Rehling P., and Pfanner N. (2007), Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain. J. Cell. Biol. 179, (6), 1115-1122

2006

Meinecke, M., Wagner, R., Kovermann, P., Guiard, B., Mick, D.U., Hutu, D.P., Voos, W., Truscott, K.N., Chacinska, A., Pfanner, N., and Rehling, P. (2006), Tim50 Maintains the Permeability Barrier of the Mitochondrial Inner Membrane. Science 312, 1523-1526

Frazier, A.E., Taylor, R., Mick, D.U., Warscheid, B., Stoepel, N., Meyer, H.E., Ryan, M.T., Guiard B., and Rehling, P. (2006), Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machinery. J. Cell Biol. 172, 553-564

Van der Laan, M., Wiedemann, N., Mick, D.U., Guiard, B., Rehling, P., and Pfanner, N. (2006), A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr. Biol. 16, 2271-2276

van der Laan, M., Rissler, M., and Rehling, P. (2006), Presequence translocase of the inner mitochondrial membrane – an intricate and highly dynamic molecular machine. FEMS Yeast Res. 6, 849-861

Albrecht, R., Rehling, P., Chacinska, A., Brix, J., Cadamuro, S.A., Volkmer, R., Guiard, B., Pfanner, N. and Zeth, K. (2006), The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. 7, 1233-1238

Wiedemann, N., Pfanner, N., and Rehling, P. (2006), Import of precursor proteins into isolated yeast mitochondria. Methods Mol. Biol. 313, 373-384

2005

Van der Laan, M., Chacinska, A., Lind, M., Perschil, I., Sickmann, A., Meyer, H.E., Guiard B., Meisinger, C., Pfanner, N., and Rehling, P. (2005), Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol. Cell. Biol. 25, 7449-7458

Brandner, K., Mick, D.U., Frazier, A.E., Taylor, R.D., Meisinger, C., and Rehling, P. (2005), Taz1, an Outer Mitochondrial Membrane Protein, Affects Stability and Assembly of Inner Membrane Protein Complexes – Implications for Barth Syndrome. Mol. Biol. Cell. 16, 5202-5214.

Chacinska, A., Lind, M., Frazier, A.E., Dudek, J., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling P. (2005), Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829

Brandner, K., Rehling, P., and Truscott, K.N. (2005), The carboxy-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase. J. Biol. Chem. 280, 6215-6221

2004

Frazier, A.E., Dudek, J., Guiard, B., Voos, W., Li, Y., Lind, M., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Bilanchone, V., Cumsky, M.G., Truscott, K.N., Pfanner, N., and Rehling, P. (2004), Pam16 plays an essential role in the presequence translocase-associated protein import motor of mitochondria. Nature Struct. Mol. Biol. 11, 226-233

Rehling, P., Brandner, K., and Pfanner, N. (2004), Mitochondrial import and the twin-pore translocase. Nature Rev. Mol. Cell Biol. 5, 519-530

Li, Y., Dudek, J., Guiard, B., Pfanner, N., Rehling, P., and Voos, W. (2004), The presequence translocase-associated protein import motor of mitochondria. J. Biol. Chem. 11, 38047-38054

Chacinska, A., and Rehling, P. (2004), Moving proteins across and in outer and inner membranes of mitochondria. Biochem. Soc. Trans. 32, 774-776

2003

Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H.E., Schönfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., and Meisinger, C. (2003), The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. USA. 100, 13207-13212

Truscott, K.N., Voos, W., Frazier, A., Lind, M., Li, Y., Geissler, A., Dudek, J., Müller, H., Sickmann, A., Meyer, H.E., Meisinger, C., Guiard, B., Rehling, P., and Pfanner, N. (2003), A J-protein is an essential subunit of the presequence translocase associated import motor of mitochondria. J. Cell Biol. 163, 707-713

Rehling, P., Model, K., Brandner, K., Kovermann, P., Sickmann, A., Meyer, H.E., Kühlbrandt, W., Wagner, R., Truscott, K.N., and Pfanner, N. (2003), Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science 299, 1747-1751

Frazier, A.E., Chacinska, A., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2003), Mitochondria use different mechanisms for transport of multispanning membrane proteins through the intermembrane space. Mol. Cell. Biol. 23, 7818-7828

Rehling, P., Pfanner, N., and Meisinger, C. (2003), Insertion of Hydrophobic Membrane Proteins into the Mitochondrial Inner Membrane – A Guided Tour. J. Mol. Biol. 326, 639-657

Chacinska, A., Rehling, P., Guiard, B., Frazier A.E., Schulze-Specking, A., Pfanner, N., Voos, W., and Meisinger, C. (2003), Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 22, 5370-5381

Agne, B., Meindl, N.M., Niederhoff, K., Einwächter, H., Rehling, P., Sickmann, A., Meyer, H.E., Girzalsky, W., and Kunau, W.-H. (2003), Pex8p: An intraperoxisomal organizer of two core complexes of the peroxisomal import machinery. Mol. Cell 11, 635-646

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