Publications (since 2004)

2023
Bohnsack KE, Yi S, Venus S, Jankowsky E, Bohnsack MT (2023) Cellular functions of eukaryotic RNA helicases and their links to human diseases. Nat Rev Mol Cell Biol , .

Wang C, Ulryck N, Herzel L, Pythoud N, Kleiber N, Guérineau V, Jactel V, Moritz C, Bohnsack MT, Carapito C, Touboul D, Bohnsack KE, Graille M (2023) N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing. Nucleic Acids Res , .

Schuhmacher JS, Tom Dieck S, Christoforidis S, Landerer C, Davila Gallesio J, Hersemann L, Seifert S, Schäfer R, Giner A, Toth-Petroczy A, Kalaidzidis Y, Bohnsack KE, Bohnsack MT, Schuman EM, Zerial M (2023) The Rab5 effector FERRY links early endosomes with mRNA localization. Mol Cell 83, 1839-1855.e13.

Khreiss A, Bohnsack KE, Bohnsack MT (2023) Molecular functions of RNA helicases during ribosomal subunit assembly. Biol Chem , .

Jia J, Hilal T, Bohnsack KE, Chernev A, Tsao N, Bethmann J, Arumugam A, Parmely L, Holton N, Loll B, Mosammaparast N, Bohnsack MT, Urlaub H, Wahl MC (2023) Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex. Nat Commun 14, 1886.

Castro-Hernández R, Berulava T, Metelova M, Epple R, Peña Centeno T, Richter J, Kaurani L, Pradhan R, Sakib MS, Burkhardt S, Ninov M, Bohnsack KE, Bohnsack MT, Delalle I, Fischer A (2023) Conserved reduction of m(6)A RNA modifications during aging and neurodegeneration is linked to changes in synaptic transcripts. Proc Natl Acad Sci U S A 120, e2204933120.

Khreiss A, Capeyrou R, Lebaron S, Albert B, Bohnsack KE, Bohnsack MT, Henry Y, Henras AK, Humbert O (2023) The DEAD-box protein Dbp6 is an ATPase and RNA annealase interacting with the peptidyl transferase center (PTC) of the ribosome. Nucleic Acids Res , .

2022
Chattopadhyay S, Garcia-Martinez J, Haimovich G, Fischer J, Khwaja A, Barkai O, Chuartzman SG, Schuldiner M, Elran R, Rosenberg MI, Urim S, Deshmukh S, Bohnsack KE, Bohnsack MT, Perez-Ortin JE, Choder M (2022) RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway. Nat Commun 13, 7184.

Bohnsack KE, Henras AK, Nielsen H, Bohnsack MT (2022) Making ends meet: a universal driver of large ribosomal subunit biogenesis. Trends Biochem Sci , .

Bohnsack KE, Kanwal N, Bohnsack MT (2022) Prp43/DHX15 exemplify RNA helicase multifunctionality in the gene expression network. Nucleic Acids Res , .

Bohnsack KE, Kleiber N, Lemus-Diaz N, Bohnsack MT (2022) Roles and dynamics of 3-methylcytidine in cellular RNAs. Trends Biochem Sci , .

Kleiber N, Lemus-Diaz N, Stiller C, Heinrichs M, Mai MM, Hackert P, Richter-Dennerlein R, Höbartner C, Bohnsack KE, Bohnsack MT (2022) The RNA methyltransferase METTL8 installs m³C₃₂ in mitochondrial tRNAs^Thr/Ser(UCN) to optimise tRNA structure and mitochondrial translation. Nat Commun 13, 209.

2021
Aquino GRR, Hackert P, Krogh N, Pan KT, Jaafar M, Henras AK, Nielsen H, Urlaub H, Bohnsack KE, Bohnsack MT (2021) The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly. Nat Commun 12, 6152.

Jaafar M, Contreras J, Dominique C, Martín-Villanueva S, Capeyrou R, Vitali P, Rodríguez-Galán O, Velasco C, Humbert O, Watkins NJ, Villalobo E, Bohnsack KE, Bohnsack MT, Henry Y, Merhi RA, de la Cruz J, Henras AK (2021) Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast. Nat Commun 12, 6153.

Cruz-Zaragoza LD, Dennerlein S, Linden A, Yousefi R, Lavdovskaia E, Aich A, Falk RR, Gomkale R, Schöndorf T, Bohnsack MT, Richter-Dennerlein R, Urlaub H, Rehling P (2021) An in vitro system to silence mitochondrial gene expression. Cell 184, 5824-5837.

Birikmen M, Bohnsack KE, Tran V, Somayaji S, Bohnsack MT, Ebersberger I (2021) Tracing Eukaryotic Ribosome Biogenesis Factors Into the Archaeal Domain Sheds Light on the Evolution of Functional Complexity. Front Microbiol 12, 739000.

Bohnsack KE, Ficner R, Bohnsack MT, Jonas S (2021) Regulation of DEAH-box RNA helicases by G-patch proteins. Biol Chem 402, 561-579.

Aquino GRR, Krogh N, Hackert P, Martin R, Gallesio JD, van Nues RW, Schneider C, Watkins NJ, Nielsen H, Bohnsack KE, Bohnsack MT (2021) RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2′-O-methylation. Nucleic Acids Res 49, 4066-4084.

Popova B, Wang D, Pätz C, Akkermann D, Lázaro DF, Galka D, Kolog Gulko M, Bohnsack MT, Möbius W, Bohnsack KE, Outeiro TF, Braus GH (2021) DEAD-box RNA helicase Dbp4/DDX10 is an enhancer of α-synuclein toxicity and oligomerization. PLoS Genet 17, e1009407.

Choudhury P, Kretschmer J, Hackert P, Bohnsack KE, Bohnsack MT (2021) The DExD box ATPase DDX55 is recruited to domain IV of the 28S ribosomal RNA by its C-terminal region. RNA Biol 18, 1124-1135.

2020
Jia J, Absmeier E, Holton N, Pietrzyk-Brzezinska AJ, Hackert P, Bohnsack KE, Bohnsack MT, Wahl MC (2020) The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations. Nat Commun 11, 5535.

Lemus-Diaz N, Ferreira RR, Bohnsack KE, Gruber J, Bohnsack MT (2020) The human box C/D snoRNA U3 is a miRNA source and miR-U3 regulates expression of sortin nexin 27. Nucleic Acids Res 48, 8074-8089.

Davila Gallesio J, Hackert P, Bohnsack KE, Bohnsack MT (2020) Sgd1 is an MIF4G domain-containing cofactor of the RNA helicase Fal1 and associates with the 5′ domain of the 18S rRNA sequence. RNA Biol 17, 539-553.

Berulava T, Buchholz E, Elerdashvili V, Pena T, Islam MR, Lbik D, Mohamed BA, Renner A, von Lewinski D, Sacherer M, Bohnsack KE, Bohnsack MT, Jain G, Capece V, Cleve N, Burkhardt S, Hasenfuss G, Fischer A, Toischer K (2020) Changes in m6A RNA methylation contribute to heart failure progression by modulating translation. Eur J Heart Fail 22, 54-66.

Braun CM, Hackert P, Schmid CE, Bohnsack MT, Bohnsack KE, Perez-Fernandez J (2020) Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit. Nucleic Acids Res 48, 405-420.

2019
Bohnsack KE, Bohnsack MT (2019) RNA-Binding Proteins Chaperone Ribonucleoprotein Complex Assembly to Solve the RNA-Folding Problem. Cell 179, 1248-1250.

van Tran N, Ernst FGM, Hawley BR, Zorbas C, Ulryck N, Hackert P, Bohnsack KE, Bohnsack MT, Jaffrey SR, Graille M, Lafontaine DLJ (2019) The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112. Nucleic Acids Res 47, 7719-7733.

Bohnsack KE, Bohnsack MT (2019) Uncovering the assembly pathway of human ribosomes and its emerging links to disease. EMBO J 38, e100278.

Bohnsack KE, Höbartner C, Bohnsack MT (2019) Eukaryotic 5-methylcytosine (m⁵C) RNA Methyltransferases: Mechanisms, Cellular Functions, and Links to Disease. Genes (Basel) 10, 102.

Choudhury P, Hackert P, Memet I, Sloan KE, Bohnsack MT (2019) The human RNA helicase DHX37 is required for release of the U3 snoRNP from pre-ribosomal particles. RNA Biol 16, 54-68.

2018
Brüning L, Hackert P, Martin R, Davila Gallesio J, Aquino GRR, Urlaub H, Sloan KE, Bohnsack MT (2018) RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes. Nat Commun 9, 5383.

Sednev MV, Mykhailiuk V, Choudhury P, Halang J, Sloan KE, Bohnsack MT, Höbartner C (2018) N⁶ -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes. Angew Chem Int Ed Engl 57, 15117-15121.

Kretschmer J, Rao H, Hackert P, Sloan KE, Höbartner C, Bohnsack MT (2018) The m⁶A reader protein YTHDC2 interacts with the small ribosomal subunit and the 5′-3′ exoribonuclease XRN1. RNA 24, 1339-1350.

Bohnsack MT, Sloan KE (2018) Modifications in small nuclear RNAs and their roles in spliceosome assembly and function. Biol Chem 399, 1265-1276.

Aksu M, Pleiner T, Karaca S, Kappert C, Dehne HJ, Seibel K, Urlaub H, Bohnsack MT, Görlich D (2018) Xpo7 is a broad-spectrum exportin and a nuclear import receptor. J Cell Biol 217, 2329-2340.

Sloan KE, Bohnsack MT (2018) Unravelling the Mechanisms of RNA Helicase Regulation. Trends Biochem Sci 43, 237-250.

Bohnsack MT, Sloan KE (2018) The mitochondrial epitranscriptome: the roles of RNA modifications in mitochondrial translation and human disease. Cell Mol Life Sci 75, 241-260.

2017
Warda AS, Kretschmer J, Hackert P, Lenz C, Urlaub H, Höbartner C, Sloan KE, Bohnsack MT (2017) Human METTL16 is a N⁶-methyladenosine (m⁶A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep 18, 2004-2014.

Jungfleisch J, Nedialkova DD, Dotu I, Sloan KE, Martinez-Bosch N, Brüning L, Raineri E, Navarro P, Bohnsack MT, Leidel SA, Díez J (2017) Corrigendum: A novel translational control mechanism involving RNA structures within coding sequences. Genome Res 27, 95-106.

Haag S, Kretschmer J, Sloan KE, Bohnsack MT (2017) Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo. Methods Mol Biol 1562, 269-281.

Haag S, Sloan KE, Höbartner C, Bohnsack MT (2017) In Vitro Assays for RNA Methyltransferase Activity. Methods Mol Biol 1562, 259-268.

Memet I, Doebele C, Sloan KE, Bohnsack MT (2017) The G-patch protein NF-κB-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis. Nucleic Acids Res 45, 5359-5374.

Sloan KE, Warda AS, Sharma S, Entian KD, Lafontaine DLJ, Bohnsack MT (2017) Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function. RNA Biol 14, 1138-1152.

Jungfleisch J, Nedialkova DD, Dotu I, Sloan KE, Martinez-Bosch N, Brüning L, Raineri E, Navarro P, Bohnsack MT, Leidel SA, Díez J (2017) A novel translational control mechanism involving RNA structures within coding sequences. Genome Res 27, 95-106.

Sloan KE, Höbartner C, Bohnsack MT (2017) How RNA modification allows non-conventional decoding in mitochondria. Cell Cycle 16, 145-146.

2016
Warda AS, Freytag B, Haag S, Sloan KE, Görlich D, Bohnsack MT (2016) Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment. Hum Mol Genet 25, 5353-5364.

Haag S, Sloan KE, Ranjan N, Warda AS, Kretschmer J, Blessing C, Hübner B, Seikowski J, Dennerlein S, Rehling P, Rodnina MV, Höbartner C, Bohnsack MT (2016) NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation. EMBO J 35, 2104-2119.

Heininger AU, Hackert P, Andreou AZ, Boon KL, Memet I, Prior M, Clancy A, Schmidt B, Urlaub H, Schleiff E, Sloan KE, Deckers M, Lührmann R, Enderlein J, Klostermeier D, Rehling P, Bohnsack MT (2016) Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol 13, 320-330.

Sloan KE, Gleizes PE, Bohnsack MT (2016) Nucleocytoplasmic Transport of RNAs and RNA-Protein Complexes. J Mol Biol 428, 2040-2059.

2015
Haag S, Warda AS, Kretschmer J, Günnigmann MA, Höbartner C, Bohnsack MT (2015) NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs. RNA 21, 1532-1543.

Weis BL, Palm D, Missbach S, Bohnsack MT, Schleiff E (2015) atBRX1-1 and atBRX1-2 are involved in an alternative rRNA processing pathway in Arabidopsis thaliana. RNA 21, 415-425.

Haag S, Kretschmer J, Bohnsack MT (2015) WBSCR22/Merm1 is required for late nuclear pre-ribosomal RNA processing and mediates N7-methylation of G1639 in human 18S rRNA. RNA 21, 180-187.

Sloan KE, Leisegang MS, Doebele C, Ramírez AS, Simm S, Safferthal C, Kretschmer J, Schorge T, Markoutsa S, Haag S, Karas M, Ebersberger I, Schleiff E, Watkins NJ, Bohnsack MT (2015) The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21. Nucleic Acids Res 43, 553-564.

2014
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E (2014) The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana. Plant J 80, 1043-1056.

Martin R, Hackert P, Ruprecht M, Simm S, Brüning L, Mirus O, Sloan KE, Kudla G, Schleiff E, Bohnsack MT (2014) A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase. RNA 20, 1173-1182.

Sloan KE, Bohnsack MT, Schneider C, Watkins NJ (2014) The roles of SSU processome components and surveillance factors in the initial processing of human ribosomal RNA. RNA 20, 540-550.

Ebersberger I, Simm S, Leisegang MS, Schmitzberger P, Mirus O, von Haeseler A, Bohnsack MT, Schleiff E (2014) The evolution of the ribosome biogenesis pathway from a yeast perspective. Nucleic Acids Res 42, 1509-1523.

2013
Sloan KE, Bohnsack MT, Watkins NJ (2013) The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress. Cell Rep 5, 237-247.

Missbach S, Weis BL, Martin R, Simm S, Bohnsack MT, Schleiff E (2013) 40S ribosome biogenesis co-factors are essential for gametophyte and embryo development. PLoS One 8, e54084.

Martin R, Straub AU, Doebele C, Bohnsack MT (2013) DExD/H-box RNA helicases in ribosome biogenesis. RNA Biol 10, 4-18.

2012
Leisegang MS, Martin R, Ramírez AS, Bohnsack MT (2012) Exportin t and Exportin 5: tRNA and miRNA biogenesis – and beyond. Biol Chem 393, 599-604.

Bohnsack MT, Tollervey D, Granneman S (2012) Identification of RNA helicase target sites by UV cross-linking and analysis of cDNA. Methods Enzymol 511, 275-288.

Veith T, Martin R, Wurm JP, Weis BL, Duchardt-Ferner E, Safferthal C, Hennig R, Mirus O, Bohnsack MT, Wöhnert J, Schleiff E (2012) Structural and functional analysis of the archaeal endonuclease Nob1. Nucleic Acids Res 40, 3259-3274.

Watkins NJ, Bohnsack MT (2012) The box C/D and H/ACA snoRNPs: key players in the modification, processing and the dynamic folding of ribosomal RNA. Wiley Interdiscip Rev RNA 3, 397-414.

Veith T, Wurm JP, Duchardt-Ferner E, Weis B, Martin R, Safferthal C, Bohnsack MT, Schleiff E, Wöhnert J (2012) Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis. Biomol NMR Assign 6, 47-50.

2011
Lehmann P, Bohnsack MT, Schleiff E (2011) The functional domains of the chloroplast unusual positioning protein 1. Plant Sci 180, 650-654.

Meyer B, Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD (2011) The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. Nucleic Acids Res 39, 1526-1537.

2010
Bohnsack MT, Schleiff E (2010) The evolution of protein targeting and translocation systems. Biochim Biophys Acta 1803, 1115-1130.

2009
Bohnsack MT, Martin R, Granneman S, Ruprecht M, Schleiff E, Tollervey D (2009) Prp43 bound at different sites on the pre-rRNA performs distinct functions in ribosome synthesis. Mol Cell 36, 583-592.

Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE (2009) Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects. Hum Mol Genet 18, 3615-3625.

2008
Bohnsack MT, Kos M, Tollervey D (2008) Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase. EMBO Rep 9, 1230-1236.

Ciais D, Bohnsack MT, Tollervey D (2008) The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3′ processing pathway. Nucleic Acids Res 36, 3075-3084.

Leulliot N, Bohnsack MT, Graille M, Tollervey D, Van Tilbeurgh H (2008) The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Nucleic Acids Res 36, 629-639.

2007
Dumont J, Petri S, Pellegrin F, Terret ME, Bohnsack MT, Rassinier P, Georget V, Kalab P, Gruss OJ, Verlhac MH (2007) A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes. J Cell Biol 176, 295-305.

Bredemeier R, Schlegel T, Ertel F, Vojta A, Borissenko L, Bohnsack MT, Groll M, von Haeseler A, Schleiff E (2007) Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. J Biol Chem 282, 1882-1890.

2006
Ribbeck K, Groen AC, Santarella R, Bohnsack MT, Raemaekers T, Köcher T, Gentzel M, Görlich D, Wilm M, Carmeliet G, Mitchison TJ, Ellenberg J, Hoenger A, Mattaj IW (2006) NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubules. Mol Biol Cell 17, 2646-2660.

Bohnsack MT, Stüven T, Kuhn C, Cordes VC, Görlich D (2006) A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes. Nat Cell Biol 8, 257-263.

2004
Mingot JM, Bohnsack MT, Jäkle U, Görlich D (2004) Exportin 7 defines a novel general nuclear export pathway. EMBO J 23, 3227-3236.

Bohnsack MT, Czaplinski K, Gorlich D (2004) Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA 10, 185-191.